Phenotypic characterization of encephalitis in the brains of goats experimentally infected with Spanish Goat Encephalitis Virus.

Spanish goat encephalitis virus (SGEV) is a novel tick–borne flavivirus subtype, closely related to the flavivirus louping ill virus (LIV). SGEV caused a severe, acute and mortal neurological disease outbreak in northern Spain in a goat herd. In order to characterize the cell population in lesions and to determine the distribution of the inflammatory cells, central nervous system (CNS) samples of nine female Alpine goats challenged subcutaneously with SGEV over the right thorax behind the elbow were evaluated using immunohistochemistry (microglia-Iba1, T lymphocytes-CD3, B lymphocytes-CD20 and astrocytes-GFAP).

The number of microglia (37.8 %) and T lymphocytes (21.5 %) was greater than the number of B lymphocytes (16.8 %). Goats were classified into clusters based on the severity of histological lesions in CNS (A-mild to moderate lesions and B-severe lesions). Microglia was significantly more abundant than T and B lymphocytes in cluster B (severe lesions). The total area occupied by glial foci revealed that medulla oblongata and spinal cord were the most affected tissues.

Astrogliosis (GFAP+) was present in the majority of the CNS sections being near to the pial surface. The lesion predominance on the right side of the medulla oblongata, which could be associated to the site of challenge suggestive of neurotropic route was also statistically confirmed. Results suggest that the cellular immune response would be the most important response to the SGEV infection.

DiagNano Organic Gold Nanorods, diameter 40 nm, absorption max 650 nm
OR-40-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Amine Gold Nanorods, diameter 25 nm, absorption max 650 nm
RFA-25-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Amine Gold Nanorods, diameter 40 nm, absorption max 650 nm
RFA-40-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Biotin Gold Nanorods, diameter 25 nm, absorption max 650 nm
RFB-25-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Biotin Gold Nanorods, diameter 40 nm, absorption max 650 nm
RFB-40-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Carboxyl Gold Nanorods, diameter 25 nm, absorption max 650 nm
RFC-25-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Carboxyl Gold Nanorods, diameter 40 nm, absorption max 650 nm
RFC-40-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Methyl Gold Nanorods, diameter 40 nm, absorption max 650 nm
RFM-40-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano NHS Gold Nanorods, diameter 25 nm, absorption max 650 nm
RFN-25-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano NHS Gold Nanorods, diameter 40 nm, absorption max 650 nm
RFN-40-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Galactose conjugated Gold Nanorods, diameter 25 nm, absorption max 650 nm
RCG-25-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Galactose conjugated Gold Nanorods, diameter 40 nm, absorption max 650 nm
RCG-40-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 25 nm, absorption max 650 nm
RCN-25-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 40 nm, absorption max 650 nm
RCN-40-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Streptavidin conjugated Gold Nanorods, diameter 25 nm, absorption max 650 nm
RCS-25-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Fluorophore Labeled Gold Nanorods, diameter 25 nm, absorption max 650 nm
RFL-25-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Fluorophore Labeled Gold Nanorods, diameter 40 nm, absorption max 650 nm
RFL-40-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano GSH conjugated Gold Nanorods, diameter 40 nm, absorption max 650 nm
RGS-40-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Protein A conjugated Gold Nanorods, diameter 25 nm, absorption max 650 nm
RCA-25-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Protein A conjugated Gold Nanorods, diameter 40 nm, absorption max 650 nm
RCA-40-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Gold Nanorods Covalent Conjugation Kit, diameter 25 nm, absorption max 650 nm
RCK-25-650	Creative Diagnostics	1 kit	EUR 1043

DiagNano Gold Nanorods Covalent Conjugation Kit, diameter 40 nm, absorption max 650 nm
RCK-40-650	Creative Diagnostics	1 kit	EUR 1043

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 25 nm, absorption max 650 nm
RMG-25-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 40 nm, absorption max 650 nm
RMG-40-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 25 nm, absorption max 650 nm
RRG-25-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 40 nm, absorption max 650 nm
RRG-40-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 25 nm, absorption max 650 nm
RHA-25-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 40 nm, absorption max 650 nm
RHA-40-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 25 nm, absorption max 650 nm
RHG-25-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 40 nm, absorption max 650 nm
RHG-40-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 25 nm, absorption max 650 nm
RHM-25-650	Creative Diagnostics	1 mL	EUR 1022

DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 40 nm, absorption max 650 nm
RHM-40-650	Creative Diagnostics	1 mL	EUR 1022

MitoViewâ„¢ 650
9-70075	Biotium	EUR 220.00 EUR 93.00	20X50UG 50UG
Description: Minimum order quantity: 1 unit of 50UG

Cyanine7 tetrazine, 100 mg
650	Lumiprobe	100 mg	EUR 1426

NucSpot Live 650
40082	Biotium	50uL	EUR 355
Description: Minimum order quantity: 1 unit of 50uL

NucSpot Live 650
40082-T	Biotium	10uL	EUR 116
Description: Minimum order quantity: 1 unit of 10uL

CytoTell™ Red 650
22255	AAT Bioquest	500 Tests	EUR 132

CytoTell™ Red 650
22256	AAT Bioquest	1000 Tests	EUR 176

AR (Ab-650) Antibody
CSB-PA227367-	Cusabio		EUR 335
Description: A polyclonal antibody against AR (Ab-650). Recognizes AR (Ab-650) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

AR (Ab-650) Antibody
CSB-PA227367-100ul	Cusabio	100ul	EUR 316
Description: A polyclonal antibody against AR (Ab-650). Recognizes AR (Ab-650) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

Streptavidin Tag 650 50µg
ABP-NAB-SA650	Allele Biotech	50µg	Ask for price

hsa-miR-650 Primers
MPH01952	ABM	150 ul / 10 uM	EUR 121

Polyclonal DGKZ Antibody (aa601-650)
APR03008G	Leading Biology	0.05ml	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human DGKZ (aa601-650). This antibody is tested and proven to work in the following applications:

Androgen Receptor (Ab-650) Antibody
21105-100ul	SAB	100ul	EUR 252

Androgen Receptor (Ab-650) Antibody
21105-50ul	SAB	50ul	EUR 187

hsa-miR-650 miRNA Inhibitor
MIH03308	ABM	2 x 5.0 nmol	EUR 176

hsa-miR-650 miRNA Antagomir
MNH03308	ABM	2 x 5.0 nmol	EUR 329

Cal Red™ R525/650 AM
20590	AAT Bioquest	1 mg	EUR 480

Cal Red™ R525/650 AM
20591	AAT Bioquest	10x50 ug	EUR 350

Polyclonal DUSP16 / MKP7 Antibody (aa600-650)
AMM06968G	Leading Biology	0.05mg	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human DUSP16 / MKP7 (aa600-650). This antibody is tested and proven to work in the following applications:

ASB-650 Antibody Conjugation Kit (OKCC00005)
OKCC00005	Aviva Systems Biology	1 Unit	EUR 213
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

BDP 630/650 tetrazine, 1 mg
154	Lumiprobe	1 mg	EUR 108

BDP 630/650 azide, 1 mg
15430	Lumiprobe	1 mg	EUR 108

BDP 630/650 hydrazide, 1 mg
15470	Lumiprobe	1 mg	EUR 108

BDP 630/650 maleimide, 1 mg
15480	Lumiprobe	1 mg	EUR 108

BDP 630/650 amine, 1 mg
154C0	Lumiprobe	1 mg	EUR 108

BDP 650/665 azide, 1 mg
18430	Lumiprobe	1 mg	EUR 108

BDP 650/665 maleimide, 1 mg
18480	Lumiprobe	1 mg	EUR 108

BDP 630/650 tetrazine, 100 mg
654	Lumiprobe	100 mg	EUR 1426

BDP 630/650 hydrazide, 100 mg
65470	Lumiprobe	100 mg	EUR 839

BDP 630/650 maleimide, 100 mg
65480	Lumiprobe	100 mg	EUR 839

BDP 630/650 amine, 100 mg
654C0	Lumiprobe	100 mg	EUR 839

BDP 650/665 maleimide, 100 mg
68480	Lumiprobe	100 mg	EUR 839

BDP 630/650 tetrazine, 5 mg
254	Lumiprobe	5 mg	EUR 234

BDP 630/650 azide, 5 mg
25430	Lumiprobe	5 mg	EUR 182

BDP 630/650 hydrazide, 5 mg
25470	Lumiprobe	5 mg	EUR 182

BDP 630/650 maleimide, 5 mg
25480	Lumiprobe	5 mg	EUR 182

BDP 630/650 amine, 5 mg
254C0	Lumiprobe	5 mg	EUR 182

BDP 650/665 azide, 5 mg
28430	Lumiprobe	5 mg	EUR 182

BDP 650/665 maleimide, 5 mg
28480	Lumiprobe	5 mg	EUR 182

BDP 630/650 alkyne, 1 mg
A54B0	Lumiprobe	1 mg	EUR 108

BDP 650/665 alkyne, 1 mg
A84B0	Lumiprobe	1 mg	EUR 108

BDP 630/650 alkyne, 5 mg
B54B0	Lumiprobe	5 mg	EUR 182

BDP 650/665 alkyne, 5 mg
B84B0	Lumiprobe	5 mg	EUR 182

BDP 630/650 alkyne, 10 mg
C54B0	Lumiprobe	10 mg	EUR 245

BDP 650/665 alkyne, 10 mg
C84B0	Lumiprobe	10 mg	EUR 245

BDP 630/650 alkyne, 25 mg
D54B0	Lumiprobe	25 mg	EUR 316

BDP 650/665 alkyne, 25 mg
D84B0	Lumiprobe	25 mg	EUR 316

BDP 630/650 alkyne, 50 mg
E54B0	Lumiprobe	50 mg	EUR 501

BDP 650/665 alkyne, 50 mg
E84B0	Lumiprobe	50 mg	EUR 501

BDP 630/650 alkyne, 100 mg
F54B0	Lumiprobe	100 mg	EUR 839

BDP 650/665 alkyne, 100 mg
F84B0	Lumiprobe	100 mg	EUR 839

BDP 630/650 azide, 10 mg
35430	Lumiprobe	10 mg	EUR 245

BDP 650/665 azide, 10 mg
38430	Lumiprobe	10 mg	EUR 245

BDP 630/650 tetrazine, 25 mg
454	Lumiprobe	25 mg	EUR 513

BDP 630/650 azide, 25 mg
45430	Lumiprobe	25 mg	EUR 316

BDP 630/650 hydrazide, 25 mg
45470	Lumiprobe	25 mg	EUR 316

BDP 630/650 maleimide, 25 mg
45480	Lumiprobe	25 mg	EUR 316

BDP 630/650 amine, 25 mg
454C0	Lumiprobe	25 mg	EUR 316

BDP 650/665 azide, 25 mg
48430	Lumiprobe	25 mg	EUR 316

BDP 650/665 maleimide, 25 mg
48480	Lumiprobe	25 mg	EUR 316

BDP 630/650 tetrazine, 50 mg
554	Lumiprobe	50 mg	EUR 921

BDP 630/650 azide, 50 mg
55430	Lumiprobe	50 mg	EUR 501

BDP 630/650 hydrazide, 50 mg
55470	Lumiprobe	50 mg	EUR 501

BDP 630/650 maleimide, 50 mg
55480	Lumiprobe	50 mg	EUR 501

BDP 630/650 amine, 50 mg
554C0	Lumiprobe	50 mg	EUR 501